This paper reports nine new moss records for Mexico from the Lacandona Forest: Calymperes rubiginosum, Fissidens guianensis var. guianensis, Mniomalia viridis, Orthostichella hexasticha, Orthostichopsis praetermisa, Phyllodrepanium falcifolium, Syrrhopodon flexifolius, Syrrhopodon africanus subsp. graminicola, and Syrrhopodon hornschuchii. Findings reported here demonstrate that there is still incomplete knowledge of the moss diversity in the Lacandona tropical rain forest region. Future research in tropical forests of southeastern Mexico and Central America will help to corroborate the continuity of distributional patterns from South America to North America through the Central American bridge.